Development issue of antiprotozoal agents to treat and prevent protozoal infections of fish, and theoretical and practical approaches to its solution
نویسندگان
چکیده
The purpose of the research is to determine safe parameters for use certain fatty acid amides against protozoal diseases fish. Materials and methods . synthesis biological activity determination new compounds were studied in laboratory conditions chemical creative team North Caucasus Zonal Research Veterinary Institute. protistocidal substances was determined a liquid nutritional medium by serial dilution according our technique. ciliate Colpoda steinii used as test culture. solution toxicity on aquarium Guppy ( Poecilia reticulata ). efficacy assessed an alternative form response: minimum concentration that all protozoan species died considered protistocidal; maximum fish remained alive recognized fish, i.e., first after absolute lethal concentrations. Compliance with these required prepared solutions having minimal ranges from 1 0.1 µg/mL, well continuous monitoring recording results. Two tested, namely, myristic amide oleic amide. Results discussion viable aqueous 0.2 μg/mL 3 days (monitoring period), 3.5 hours or more (up 20 hours) 0.5 μg/mL. At same time, 0.14 had effect C. if exposed 40 minutes. tolerated µg/mL 2 4 h. level 0.58 at 18 h exposure.
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ژورنال
عنوان ژورنال: Rossii?skii? Parazitologicheskii? Zhurnal
سال: 2022
ISSN: ['1998-8435', '2541-7843']
DOI: https://doi.org/10.31016/1998-8435-2022-16-3-367-376